Part:BBa_K217000

cmv_tat_GFP

The part is controlled by a CMV (cytomegaolvirus) promoter. This promoter is constitutive (always on). Controlled by the promoter is a tat peptide sequence connected to GFP. Tat is a cell-penetrating peptide found in HIV-1. This fusion protein should be handled as Biosafety Level 2 because tat has the ability to cross cell membranes. Proper protocol includes covering exposed skin, goggles, and working in a proper microbiological hood. For more safety information, please see this [http://ors.bsd.uchicago.edu/IBC/resources/tatProtein.html tat Safety Page] from the University of Chicago.

Essentially, the cell that has this plasmid is will shoot out green "bullets" of GFP into its surroundings (and into itself).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 79
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 79
  Illegal NheI site found at 10
  Illegal NotI site found at 850
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 79
  Illegal BamHI site found at 66
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 79
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 79
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 728